Surface modification of PLGA nanospheres with Gd-DTPA and Gd-DOTA for high-relaxivity MRI contrast agents

Autor(en)
Gerda Ratzinger-Stöger, Prashant Agrawal, Wilfried Körner, Julia Lonkai, Honorius M. H. F. Sanders, Enzo Terreno, Michael Wirth, Gustav J. Strijkers, Klaas Nicolay, Franz Gabor
Abstrakt

The preparation of particulate contrast agents for magnetic resonance imaging (MRI) based on biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanocarriers is reported. By spacer-aided covalent surface-grafting of the prominent chelating ligands diethylenetriaminepentaacetic acid (DTPA) and 1,4,7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), respectively, up to 236 mu g gadolinium per mg PLGA can be immobilized in a stable manner. Due to the localisation at the particle surface, water protons may effectively interact with the gadolinium chelates and the modified particles exhibit high proton relaxivities as confirmed by T1 relaxivities of up to 17.5 mM(-1)s(-1) (25 degrees C, 1.41 T) in case of Gd-DOTA-functionalized carriers and also supported by NMRD profiles. The obtained values compare favorably with marketed low-molecular weight contrast agents and thus suggest suitability for in vivo use. (C) 2010 Elsevier Ltd. All rights reserved.

Organisation(en)
Externe Organisation(en)
Eindhoven University of Technology, Università degli Studi di Torino
Journal
Biomaterials
Band
31
Seiten
8716-8723
Anzahl der Seiten
8
ISSN
0142-9612
DOI
https://doi.org/10.1016/j.biomaterials.2010.07.095
Publikationsdatum
2010
Peer-reviewed
Ja
ÖFOS 2012
3012 Pharmazie, Pharmakologie, Toxikologie
Link zum Portal
https://ucrisportal.univie.ac.at/de/publications/4fff783a-b381-4a27-be50-a014ea3bddfe