IMPACT OF PARTICLE SIZE AND LIGHT EXPOSURE ON THE EFFECTS OF TiO2 NANOPARTICLES ON CAENORHABDITIS ELEGANS

Autor(en)

Judith S. Angelstorf, Wolfgang Ahlf, Frank von der Kammer, Susanne Heise

Abstrakt

The increasing use of engineered nanoparticles in industrial and consumer products leads to a release of the anthropogenic
contaminants to the aquatic environment. To obtain a better understanding of the environmental effects of these particles, the nematode
Caenorhabditis elegans was used to investigate the organism-level effects and in vivo molecular responses. Toxicity of bulk-scale
(160 nm) and nanoscale (21 nm) titanium dioxide (TiO2) was tested under dark and light conditions, following ISO 10872.
The expression of sod-3, a mitochondrial superoxide dismutase, was quantified as an indicator for oxidative stress induced by the
photocatalytically active material. Particle sizes were estimated using dynamic light scattering and scanning electron microscopy.
Although both materials agglomerated to a comparable secondary particle size of 300 nm to 1500 nm and were ingested into the intestine,
only nanoscale-TiO2 significantly inhibited reproduction (lowest-observed-effect-concentration [LOEC]: 10 mg/L). Light exposure
induced the production of reactive oxygen species (ROS) by nanoscale-TiO2 and increased toxicity of the nanomaterial from a median
effect concentration of more than 100 mg/L to 53 mg/L. No evidence was found for inner cellular photocatalytic activity of nanoscale-
TiO2. Therefore, oxidative damage of the membranes of intestinal cells is suggested as a potential mode of action. Results highlight
the importance of primary particle size and environmental parameters on the toxicity of TiO2.

Organisation(en)

Externe Organisation(en)

Universität Hamburg, Technische Universität Hamburg-Harburg (TUHH)

Journal

Environmental Toxicology and Chemistry

Band

33

Seiten

2288-2296

Anzahl der Seiten

9

ISSN

0730-7268

DOI

https://doi.org/10.1002/etc.2674

Publikationsdatum

06-2014

Peer-reviewed

Ja

ÖFOS 2012

104023 Umweltchemie, 104002 Analytische Chemie, 105904 Umweltforschung, 210006 Nanotechnologie

Schlagwörter

ASJC Scopus Sachgebiete

Health, Toxicology and Mutagenesis, Environmental Chemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/c68f9a48-0de4-4737-aad5-24802fb49bd6