Accurate LC-ESI-MS/MS quantification of 2'-deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C ₄-labeled internal standard

Autor(en)

Yvonne Schindlegger, Eva Oburger, Barbara Gruber, Walter D. C. Schenkeveld, Stephan Krämer, Markus Puschenreiter, Gunda Koellensperger, Stephan Hann

Abstrakt

For the first time the phytosiderophore 2'-deoxymugineic acid (DMA) could be accurately quantified by LC-MS/MS in plant and soil related samples. For this purpose a novel chromatographic method employing porous graphitic carbon as stationary phase combined with ESI-MS/MS detection in selected reaction monitoring was developed. Isotope dilution was implemented by using in-house synthesized DMA as external calibrant and

¹³C

₄-labeled DMA as internal standard (concentration levels of standards 0.1-80 μM, determination coefficient of linear regression R

² > 0.9995). Sample preparation involved acidification of the samples in order to obtain complete dissociation of metal-DMA complexes. Excellent matrix related LOD and LOQ depending on different experimental setups were obtained in the range of 3-34 nM and 11-113 nM, respectively. Standard addition experiments and the implementation of the internal

¹³C

₄-DMA standard proved the accuracy of the quantification strategy even in complex matrices such as soil solution. The repeatability of the method, including sample preparation, expressed as short- and long term precision was below 4 and 5% RSD, respectively. Finally, application in the context of plant and soil research to samples from rhizosphere sampling via micro suction cups, from soil solutions and soil adsorption/extraction studies revealed a DMA concentration range from 0.1 to 235 μM.

Organisation(en)

Institut für Analytische Chemie

Externe Organisation(en)

Universität für Bodenkultur Wien

Journal

Electrophoresis

Band

35

Seiten

1375-1385

Anzahl der Seiten

11

ISSN

0173-0835

DOI

https://doi.org/10.1002/elps.201300551

Publikationsdatum

05-2014

Peer-reviewed

Ja

ÖFOS 2012

104004 Chemische Biologie, 104023 Umweltchemie, 104002 Analytische Chemie, 105906 Umweltgeowissenschaften

Schlagwörter

ASJC Scopus Sachgebiete

Analytical Chemistry, Biochemistry, Clinical Biochemistry

Link zum Portal

https://ucrisportal.univie.ac.at/de/publications/e53ea939-8ea7-4fd5-87b6-a204cdaf3076