3D characterization of natural colloids by FlFFF-MALLS-TEM

Author(s)
Mohammed Baalousha, Frank von der Kammer, M. Motelica-Heino, Philippe Le Coustumer
Abstract

Understanding the role of colloids in the environment needs a more complete characterization of their size and shape than the usually used hydrodynamic diameter. A novel methodology to determine three dimensional description of colloids has been successfully experimented in this work by coupling Flow-Field Flow Fractionation (FlFFF) to Multi Angle Laser Light Scattering (MALLS), and Transmission Electron Microscope (TEM). This methodology was evaluated for a soil colloidal sample. Results show that, the particles surface areas determined by FlFFF-MALLS-TEM, are 3-4 times greater than those calculated using the hydrodynamic diameter determined by FlFFF. Further, results indicated that, the surface area/volume values are 3-5 times greater than those calculated based on the hydrodynamic diameter. Therefore, a correction factor must be applied when the particle surface area is calculated from the hydrodynamic diameter determined by FlFFF. © Springer-Verlag 2005.

Organisation(s)
External organisation(s)
Université Bordeaux, Bureau des Recherches Geologiques et Minieres (BRGM)
Journal
Analytical and Bioanalytical Chemistry
Volume
383
Pages
549-556
No. of pages
8
ISSN
1618-2642
DOI
https://doi.org/10.1007/s00216-005-0006-9
Publication date
2006
Peer reviewed
Yes
Austrian Fields of Science 2012
104023 Environmental chemistry
Portal url
https://ucrisportal.univie.ac.at/en/publications/8f38103f-5956-4d51-8869-73e3ddc163e6