Spectroscopic analysis of sequestered chloroplasts in Elphidium williamsoni (Foraminifera)

Author(s)
Michael Lintner, Manfred Wildner, Bianca Lintner, Wolfgang Wanek, Petra Heinz
Abstract

Foraminifera are unicellular, marine organisms that occur worldwide. A very common species in the German Wadden Sea is Elphidium williamsoni. Some foraminifera (such as elphidia) are able to use kleptoplastidy, which allows them to incorporate chloroplasts from their algal food source into their own cell body. The experiments reported here are based on the fact that chlorophyll (a and c) can be detected in the intact cells with spectroscopic methods in the visible spectral range, which allows an indirect investigation of the presence of sequestered chloroplasts. Starving experiments of E. williamsoni in the light (24 h continuous) showed that the greatest decrease in chlorophyll content was recorded within the first 20-30 days. From day 60 on, chlorophyll was hardly detectable. Through subsequent feeding on a renewed algal food source a significant increase in the chlorophyll content in foraminifera was noticed. The degradation of chlorophyll in the dark (24 h continuous darkness) during the starving period was much more complex. Chlorophyll was still detected in the cells after 113 days of starving time. Therefore, we hypotheses that the effect of photoinhibition applies to chloroplasts in foraminifera under continuous illumination.

Organisation(s)
Department of Palaeontology, Department of Mineralogy and Crystallography, Department of Microbiology and Ecosystem Science
Journal
Journal of Photochemistry and Photobiology B: Biology
Volume
238
No. of pages
7
ISSN
1011-1344
DOI
https://doi.org/10.1016/j.jphotobiol.2022.112623
Publication date
01-2023
Peer reviewed
Yes
Austrian Fields of Science 2012
106021 Marine biology, 105118 Palaeontology
Keywords
ASJC Scopus subject areas
Radiation, Radiological and Ultrasound Technology, Biophysics, Radiology Nuclear Medicine and imaging
Sustainable Development Goals
SDG 14 - Life Below Water
Portal url
https://ucrisportal.univie.ac.at/en/publications/baca7940-8515-4071-8ac1-10a936ee9d77