Accurate LC-ESI-MS/MS quantification of 2'-deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a ¹³C ₄-labeled internal standard

Author(s)

Yvonne Schindlegger, Eva Oburger, Barbara Gruber, Walter D. C. Schenkeveld, Stephan Krämer, Markus Puschenreiter, Gunda Koellensperger, Stephan Hann

Abstract

For the first time the phytosiderophore 2'-deoxymugineic acid (DMA) could be accurately quantified by LC-MS/MS in plant and soil related samples. For this purpose a novel chromatographic method employing porous graphitic carbon as stationary phase combined with ESI-MS/MS detection in selected reaction monitoring was developed. Isotope dilution was implemented by using in-house synthesized DMA as external calibrant and

¹³C

₄-labeled DMA as internal standard (concentration levels of standards 0.1-80 μM, determination coefficient of linear regression R

² > 0.9995). Sample preparation involved acidification of the samples in order to obtain complete dissociation of metal-DMA complexes. Excellent matrix related LOD and LOQ depending on different experimental setups were obtained in the range of 3-34 nM and 11-113 nM, respectively. Standard addition experiments and the implementation of the internal

¹³C

₄-DMA standard proved the accuracy of the quantification strategy even in complex matrices such as soil solution. The repeatability of the method, including sample preparation, expressed as short- and long term precision was below 4 and 5% RSD, respectively. Finally, application in the context of plant and soil research to samples from rhizosphere sampling via micro suction cups, from soil solutions and soil adsorption/extraction studies revealed a DMA concentration range from 0.1 to 235 μM.

Organisation(s)

Department of Analytical Chemistry

External organisation(s)

University of Natural Resources and Life Sciences

Journal

Electrophoresis

Volume

35

Pages

1375-1385

No. of pages

11

ISSN

0173-0835

DOI

https://doi.org/10.1002/elps.201300551

Publication date

05-2014

Peer reviewed

Yes

Austrian Fields of Science 2012

104004 Chemical biology, 104023 Environmental chemistry, 104002 Analytical chemistry, 105906 Environmental geosciences

Keywords

ASJC Scopus subject areas

Analytical Chemistry, Biochemistry, Clinical Biochemistry

Portal url

https://ucrisportal.univie.ac.at/en/publications/e53ea939-8ea7-4fd5-87b6-a204cdaf3076